| 产品编号 | bs-18136R |
| 英文名称 | Lambda Light Chain Rabbit pAb |
| 中文名称 | λ轻链抗体 |
| 别 名 | IGL@; IGLC6; IGLV; IGLJ@; IGLC1_HUMAN; IGLC1; Ig lambda chain C region MGC; Ig lambda-1 chain C region; LV151_HUMAN; IGLV1-51; Ig lambda chain V-I region BL2; Ig lambda chain V-I region EPS; Ig lambda chain V-I region NEW; Ig lambda chain V-I region NIG-6 免疫球蛋白λ轻链 |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 11 kDa |
| 细胞定位 | 细胞膜 细胞外基质 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Human Lambda Light Chain: 1-50/106 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. Subcellular Location: Extracellular region, plasma membrane. Similarity: Contains 1 Ig-like (immunoglobulin-like) domain. SWISS: P01701 Gene ID: 8217 Database links: 该抗体特异识别免疫球蛋白λ轻链,可用于鉴定白血病、浆细胞瘤以及某些非霍奇金氏淋巴瘤。这些肿瘤的共同特征是只限于单个轻链的表达,因此轻链Kappa和Lamda可用于良恶性淋巴病变(反应性淋巴细胞增生和淋巴瘤)的辅助性诊断。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGLC1) Polyclonal Antibody, Unconjugated (bs-18136R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |