| 产品编号 | bs-18173R |
| 英文名称 | Intrinsic Factor Rabbit pAb |
| 中文名称 | 胃内在因子抗体 |
| 别 名 | GIF; IF; IFMH; INF; TCN3; IF_HUMAN; CBLIF; Gastric intrinsic factor; Intrinsic factor (IF | INF); IF_MOUSE; IF_RAT; |
| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | 60 |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Intrinsic Factor: 321-417/417 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008] Function: Intrinsic factor promotes absorption of the essential vitamin Cobalamin (Cbl) in the ileum by specific receptor mediated endocytosis. Defects in the gene GIF are the cause of hereditary intrinsic factor deficiency (IFD) also called congenital pernicious anemia. IFD is an autosomal recessive disorder characterized by megaloblastic anemia. Subcellular Location: Secreted Tissue Specificity: Gastric mucosa. DISEASE: Hereditary intrinsic factor deficiency (IFD) Similarity: Belongs to the eukaryotic cobalamin transport proteinsfamily. SWISS: P27352 Gene ID: 2694 Database links: Entrez Gene: 2694 Human Entrez Gene: 14603 Mouse Omim: 609342 Human SwissProt: P27352 Human SwissProt: P52787 Mouse |
| 产品图片 |
Sample:
Lane 1: Human MKN45 cell lysates
Primary: Anti-Intrinsic Factor (bs-18173R) at 1/200 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 60 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
