| 产品编号 | bs-18368R |
| 英文名称 | LRRC17 Rabbit pAb |
| 中文名称 | 富含亮氨酸重复蛋白17抗体 |
| 别 名 | P37NB; 37kDa; 4833425M04Rik; 6130400C22Rik; LRC17_HUMAN; LRRC17; LRC17_MOUSE; leucine rich repeat containing 17 |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50kDa |
| 检测分子量 | 50 |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LRRC17: 331-430/441 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
LRRC17 is a 441 amino acid protein that localizes to the cytoplasm and contains six LRR repeats. The protein is expressed at high levels in ovary, heart, pancreas, skeletal muscle, lung, and fetal kidney and lung. LRRC17 is expressed at higher levels in non-neuronal (S-type) neuroblastoma cells than in neuroblastic (N-type) neuroblastoma cells. Function: Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors. Subcellular Location: Secreted; extracellular space. Tissue Specificity: Expressed in osteoblast cell lines. Well expressed in ovary, heart, pancreas, skeletal muscle, lung, and fetal kidney and lung and only at the basal levels in the other tissues examined including adult kidney. More expressed in S-type neuroblastoma cells than in N-type neuroblastoma cells. Similarity: Contains 6 LRR (leucine-rich) repeats. Contains 2 LRRCT domains. Contains 1 LRRNT domain. SWISS: Q8N6Y2 Gene ID: 10234 Database links: Entrez Gene: 10234 Human Entrez Gene: 100050177 Horse Entrez Gene: 74511 Mouse Entrez Gene: 100340395 Rabbit SwissProt: Q8N6Y2 Human SwissProt: Q9CXD9 Mouse Unigene: 567412 Human Unigene: 439963 Mouse Unigene: 207235 Rat |
| 产品图片 |
Sample:
Spleen (Mouse) Lysate at 40 ug
Lung (Mouse) Lysate at 40 ug
Primary: Anti- LRRC17 (bs-18368R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
