| 产品编号 | bs-18504R |
| 英文名称 | ARFGAP2 Rabbit pAb |
| 中文名称 | 锌指蛋白289抗体 |
| 别 名 | ADP-ribosylation factor GTPase-activating protein 2; ARF GAP 2; ARFG2_HUMAN; FLJ14576; FLJ26000; GTPase activating protein ZNF289; GTPase-activating protein ZNF289; IRZ; Nbla10535; Zfp289; Zinc finger protein 289; Zinc finger protein 289 ID1 regulated; ZNF 289. |
| 研究领域 | 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 57 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF289/ARFGAP2: 401-500/521 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ZNF289 functions as a GTPase-activating protein (GAP) for ARF family proteins. Localizing to the cytoplasmic side of the Golgi apparatus, ZNF289 contains one ARF-GAP domain and is found associated with COP-I-coated vesicles. Function: GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Implicated in coatomer-mediated protein transport between the Golgi complex and the endoplasmic reticulum. Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes. Subunit: Interacts with the coatomer complex. Interacts with the C-terminal appendage domain of COPG1. Subcellular Location: Cytoplasm. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side. Note: Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment. Similarity: Contains 1 Arf-GAP domain. SWISS: Q8N6H7 Gene ID: 84364 Database links: Entrez Gene: 84364 Human Entrez Gene: 77038 Mouse Omim: 606908 Human SwissProt: Q8N6H7 Human SwissProt: Q99K28 Mouse Unigene: 436204 Human Unigene: 43636 Mouse Unigene: 3707 Rat |
| 产品图片 |
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti-ZNF289 (bs-18504R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 57 kD
Observed band size: 57 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
