| 产品编号 | bs-18510R |
| 英文名称 | ZNF366 Rabbit pAb |
| 中文名称 | 锌指蛋白366抗体 |
| 别 名 | DC-SCRIPT; DCSCRIPT; Dendritic cell specific transcript; FLJ39796; MGC99415; Zfp366; Zfp366 zinc finger protein 366; Zinc finger protein 366; ZN366_HUMAN; ZNF366. |
| 研究领域 | 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Chicken, Dog, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 85 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF366: 501-600/744 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ZNF366 is a 744 amino acid nuclear protein that contains eleven C2H2-type zinc fingers and is thought to function in transcriptional regulation. The gene encoding ZNF366 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome. Function: Has transcriptional repression activity. Acts as corepressor of ESR1; the function seems to involve CTBP1 and histone deacetylases. Subunit: Interacts with ESR1, NRIP1 and CTBP1. Subcellular Location: Nucleus. Similarity: Contains 11 C2H2-type zinc fingers. SWISS: Q8N895 Gene ID: 167465 Database links: Entrez Gene: 167465 Human Entrez Gene: 238803 Mouse Omim: 610159 Human SwissProt: Q8N895 Human Unigene: 370303 Human Unigene: 343207 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZNF366) Polyclonal Antibody, Unconjugated (bs-18510R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
