| 产品编号 | bs-18638R |
| 英文名称 | MAK3/C14orf35 Rabbit pAb |
| 中文名称 | 14号染色体开放阅框35抗体 |
| 别 名 | C14orf35; Chromosome 14 open reading frame 35; FLJ35355; MAK 3; Mak3p; MGC141884; MGC141885; N acetyltransferase 12(GCN5 related putative); N acetyltransferase 12; N acetyltransferase MAK3 homolog; NAT12; Putative N acetyltransferase. |
| 研究领域 | 肿瘤 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAK3/C14orf35: 251-350/362 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MAK3 belongs to the acetyltransferase family, MAK3 subfamily. It contains one N-acetyltransferase domain. It is a probable N-acetyltransferase. Function: Catalytic subunit of the N-terminal acetyltransferase C (NatC) complex. Catalyzes acetylation of the N-terminal methionine residues of peptides beginning with Met-Leu-Ala and Met-Leu-Gly. Necessary for the lysosomal localization and function of ARL8B sugeesting that ARL8B is a NatC substrate. Subunit: Component of the N-terminal acetyltransferase C (NatC) complex, which is composed of NAA35, NAA38 and NAA30. Subcellular Location: Cytoplasmic Similarity: Belongs to the acetyltransferase family. MAK3 subfamily. Contains 1 N-acetyltransferase domain. SWISS: Q147X3 Gene ID: 122830 Database links: Entrez Gene: 122830 Human Entrez Gene: 70646 Mouse SwissProt: Q147X3 Human SwissProt: Q8CES0 Mouse Unigene: 165465 Human Unigene: 275688 Mouse Unigene: 15205 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (C14orf35) Polyclonal Antibody, Unconjugated (bs-18638R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
