| 产品编号 | bs-18649R |
| 英文名称 | MAN2C1 Rabbit pAb |
| 中文名称 | 甘露糖苷酶α胞浆抗体 |
| 别 名 | alpha D mannoside mannohydrolase; alpha mannosidase 2C1; alpha mannosidase 6A8B; alpha-D-mannoside mannohydrolase; alpha-mannosidase 2C1; MA2C1_HUMAN; Man2c1; MAN6A8; MANA; MANA1; Mannosidase alpha 6A8; Mannosidase alpha A cytoplasmic; Mannosidase alpha class 2C member 1. |
| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Pig, Cow, Horse, Sheep, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 116 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAN2C1: 61-160/1040 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Misfolded glycoproteins are deglycosylated by the peptide N-glycanase during the degradation process. Free oligosaccharides released by N-glycanase are catabolized by cytosolic MAN2C1, also designated alpha-mannosidase 2C1. MAN2C1, a member of the glycosyl hydrolase 38 family, can cleave alpha 1,2-linked, alpha 1,3-linked and alpha 1,6-linked mannose residues and is stimulated by cobalt. The furanose analogs, swainsonine (SW) and 1,4-dideoxy-1,4-imino-d-mannitol (DIM), are known inhibitors of MAN2C1. The inhibition of MAN2C1 can enhance the adhesion of Jurkat T cells, showing a cytoskeletal rearrangement of the cells. Similarity: Belongs to the glycosyl hydrolase 38 family. SWISS: Q9NTJ4 Gene ID: 4123 Database links: Entrez Gene: 4123 Human Omim: 154580 Human SwissProt: Q9NTJ4 Human Unigene: 26232 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAN2C1) Polyclonal Antibody, Unconjugated (bs-18649R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
