| 产品编号 | bs-18691R |
| 英文名称 | MAT1A Rabbit pAb |
| 中文名称 | 腺苷蛋氨酸1抗体 |
| 别 名 | AdoMet; Ams; MAT; MATA1; SAMS; SAMS1; SADE; SAS; METK1_HUMAN; MAT1A; AdoMet synthase 1; Methionine adenosyltransferase 1 (MAT 1); Methionine adenosyltransferase I/III (MAT-I/III); 2.5.1.6; AMS1; METK1_MOUSE; METK1_RAT; |
| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Cow, Horse, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MAT1A: 231-330/395 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MAT1A catalyzes a two-step reaction that involves the transfer of the adenosyl moiety of ATP to methionine to form S-adenosylmethionine and tripolyphosphate, which is subsequently cleaved to PPi and Pi. S-adenosylmethionine is the source of methyl groups for most biological methylations. Mutations in this gene are associated with methionine adenosyltransferase deficiency. Function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. Subunit: Homotetramer (MAT-I) or homodimer (MAT-III). Tissue Specificity: Expressed in liver. Post-translational modifications: S-nitrosylation of Cys-120 inactivates the enzyme. DISEASE: Methionine adenosyltransferase deficiency (MATD) [MIM:250850]: An inborn error of metabolism resulting in isolated hypermethioninemia. Most patients have no clinical abnormalities, although some neurologic symptoms may be present in rare cases with severe loss of methionine adenosyltransferase activity. Note: The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the AdoMet synthase family. SWISS: Q00266 Gene ID: 610550 Database links: Entrez Gene: 610550 Human Entrez Gene: 11720 Mouse Omim: 610550 Human SwissProt: Q00266 Human SwissProt: Q91X83 Mouse Unigene: 282670 Human Unigene: 14064 Mouse Unigene: 10418 Rat |
| 产品图片 |
Tissue/cell: mouse liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-MAT1A Polyclonal Antibody, Unconjugated(bs-18691R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
