| 产品编号 | bs-18710R |
| 英文名称 | MBTD1 Rabbit pAb |
| 中文名称 | MBTD1蛋白抗体 |
| 别 名 | AA408199; AI194990; FLJ20055; FLJ42067; Hemp; mbt domain containing 1; MBT domain-containing protein 1; mbtd1; MBTD1_HUMAN; MGC126785; RP23-440P13.2; SA49P01. |
| 研究领域 | 细胞生物 信号转导 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 71 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MBTD1: 301-400/628 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The transcriptional repressing Polycomb-group (PcG) family is a part of a cellular memory system responsible for the stable inheritance of gene activity. PcG proteins assemble into multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. MBTD1 (malignant brain tumor domain containing 1) is a 628 amino acid nuclear protein that belongs to the PcG family of proteins. Existing as a monomer, MBTD1 undergoes alternative splicing events to produce three isoforms. MBTD1 contains one FCS-type zinc finger and four MBT repeats, and is encoded by a gene that maps to human chromosome 17q21.33. Chromosome 17 comprises over 2.5% of the human genome and encodes over 1,200 genes. Two key tumor suppressor genes are associated with chromosome 17, namely, p53 and BRCA1. Function: Putative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility (By similarity). Specifically binds to monomethylated and dimethylated 'Lys-20' on histone H4. Subcellular Location: Nucleus. Similarity: Contains 1 FCS-type zinc finger. Contains 4 MBT repeats. SWISS: Q05BQ5 Gene ID: 54799 Database links: Entrez Gene: 54799 Human Entrez Gene: 103537 Mouse SwissProt: Q05BQ5 Human SwissProt: Q6P5G3 Mouse Unigene: 656803 Human Unigene: 210334 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MBTD1) Polyclonal Antibody, Unconjugated (bs-18710R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
