| 产品编号 | bs-18964R |
| 英文名称 | MMP25 Rabbit pAb |
| 中文名称 | 基质金属蛋白酶25抗体 |
| 别 名 | MMP-25; MMP20; MMP20A; MMPL1; MT-MMP 6; MT-MMP6; MT6-MMP; MT6MMP; MTMMP6; F730048C11Rik; Leukolysin; MMP25_HUMAN; MMP25; Membrane-type matrix metalloproteinase 6 (MT-MMP 6 | MTMMP6); Membrane-type-6 matrix metalloproteinase (MT6-MMP | MT6MMP); 3.4.24.-; M |
| 研究领域 | 肿瘤 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 65 kDa |
| 检测分子量 | 62 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MMP25: 431-530/562 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, the protein encoded by this gene is a member of the membrane-type MMP (MT-MMP) subfamily, attached to the plasma membrane via a glycosylphosphatidyl inositol anchor. In response to bacterial infection or inflammation, the encoded protein is thought to inactivate alpha-1 proteinase inhibitor, a major tissue protectant against proteolytic enzymes released by activated neutrophils, facilitating the transendothelial migration of neutrophils to inflammatory sites. The encoded protein may also play a role in tumor invasion and metastasis through activation of MMP2. The gene has previously been referred to as MMP20 but has been renamed MMP25. [provided by RefSeq, Jul 2008] Function: May activate progelatinase A. Subcellular Location: Cell Membrane; lipid-anchor; GPI-anchor. Tissue Specificity: Expressed predominantly in leukocytes, lung and spleen. Expressed also in colon carcinoma, astrocytoma and glioblastomas. Post-translational modifications: The precursor is cleaved by a furin endopeptidase Similarity: Belongs to the peptidase M10A family. Contains 4 hemopexin repeats. SWISS: Q9NPA2 Gene ID: 64386 Database links: Entrez Gene: 64386 Human Entrez Gene: 240047 Mouse Omim: 608482 Human SwissProt: Q9NPA2 Human SwissProt: Q3U435 Mouse Unigene: 654979 Human |
| 产品图片 |
Sample:
U251(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti-MMP25 (bs-18964R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 65 kD
Observed band size: 62 kD
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
