| 产品编号 | bs-20013R |
| 英文名称 | ALPI Rabbit pAb |
| 中文名称 | 碱性磷酸酶(肠型)抗体 |
| 别 名 | PPBI_HUMAN; Intestinal-type alkaline phosphatase; EC:3.1.3.1; IAP; Intestinal alkaline phosphatase; alkaline phosphatase, intestinal; 肠道碱性磷酸酶; 肠碱性磷酸酶; 碱性磷酸酶，肠型; 肠型碱性磷酸酶; |
| 研究领域 | 肿瘤 免疫学 信号转导 激酶和磷酸酶 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALPI: 351-450/528 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Similarity: Belongs to the alkaline phosphatase family. SWISS: P09923 Gene ID: 248 Database links: Entrez Gene: 248 Human Omim: 171740 Human SwissProt: P09923 Human Unigene: 284255 Human Unigene: 37009 Human |
| 产品图片 |
Sample:
Lane 1: Human LOVO cell lysates
Lane 2: Human FHC cell lysates
Lane 3: Human SW480 cell lysates
Primary: Anti-ALPI (bs-20013R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kDa
Observed band size: 65 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
