| 产品编号 | bs-19065R |
| 英文名称 | NDST4 Rabbit pAb |
| 中文名称 | NDST4蛋白抗体 |
| 别 名 | N-HSST; N-HSST 4; NDST-4; NHSST4; 4930439H17Rik; NDST4_HUMAN; NDST4; Glucosaminyl N-deacetylase/N-sulfotransferase 4 (NDST-4); N-heparan sulfate sulfotransferase 4 (N-HSST 4); 2.8.2.8; HSST4; NDST4_MOUSE; N-deacetylase and N-sulfotransferase 4; N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 4 |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 101kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NDST4: 101-200/872 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin initiate a set of biochemical reactions, which lead to the synthesis of oligosaccharide sequences that have specific ligand binding properties (1). These reactions are catalyzed by the monomeric enzymes GlcNAc Ndeacetylase/N-sulfotransferases (NDSTs), which have two catalytic activities (1). Multiple NDST isozymes have been identified, each having unique tissue distribution and enzymatic properties (2). Phylogenetic data suggests that NDST1-4 evolved from a common ancestral gene, which diverged to give rise to two subtypes, NDST1/2 and NDST3/4 (2). NDST1, which maps to human chromosome 5q32-q33.1, shares the most homology with NDST2, which maps to human chromosome 10q22 (3,4). The least conserved amino acids between these two enzymes are found in the N-terminus/putative transmembrane regions (3). The human NDST3 and NDST4 genes are closely linked on chromosome 4, mapping to chromosome 4q25-26 and 4q26-27, respectively (2,5,6). RT-PCR analysis of various mouse tissues reveals a restricted pattern of NDST3 and NDST4 mRNA expression when compared with that of NDST1 and NDST2, which are abundantly and ubiquitously expressed (2). Function: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity Subunit: Monomer Subcellular Location: Golgi apparatus membrane; Single-pass type II membrane protein Similarity: Belongs to the sulfotransferase 1 family. NDST subfamily. SWISS: Q9H3R1 Gene ID: 64579 Database links: Entrez Gene: 64579 Human Entrez Gene: 64580 Mouse Omim: 615039 Human SwissProt: Q9H3R1 Human SwissProt: Q9EQW8 Mouse Unigene: 591700 Human Unigene: 444289 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NDST4) Polyclonal Antibody, Unconjugated (bs-19065R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
