| 产品编号 | bs-19286R |
| 英文名称 | NLRP14 Rabbit pAb |
| 中文名称 | NLRP14蛋白抗体 |
| 别 名 | CLR11.2; GC-LRR; LRR and PYD domains-containing protein 14; NACHT; NACHT, leucine rich repeat and PYD containing 14; NACHT, LRR and PYD containing protein 14; NACHT, LRR and PYD domains-containing protein 14; NAL14_HUMAN; Nalp-iota; NALP14; NLR family, pyrin domain containing 14; NLRP14; NOD5; Nucleotide-binding oligomerization domain protein 5; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 14; PAN8. |
| 研究领域 | 细胞生物 信号转导 细胞凋亡 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 125 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NLRP14: 81-180/1093 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene belongs to the NALP protein family. Members of the NALP protein family typically contain a NACHT domain, a NACHT-associated domain (NAD), a C-terminal leucine-rich repeat (LRR) region, and an N-terminal pyrin domain (PYD). This protein may play a regulatory role in the innate immune system as similar family members belong to the signal-induced multiprotein complex, the inflammasome, that activates the pro-inflammatory caspases, caspase-1 and caspase-5. [provided by RefSeq, Jul 2008] Function: May be involved in inflammation and spermatogenesis. Tissue Specificity: Testis-specific. DISEASE: In the testis, expressed mainly in A dark spermatogonia, mid and late spermatocytes and spermatids but not in mitotically active A pale and B spermatogonia. Similarity: Belongs to the NLRP family. Contains 1 DAPIN domain. Contains 11 LRR (leucine-rich) repeats. Contains 1 NACHT domain. SWISS: Q86W24 Gene ID: 338323 Database links: Entrez Gene: 338323 Human Omim: 609665 Human SwissProt: Q86W24 Human SwissProt: Q6B966 Mouse Unigene: 449637 Human Unigene: 618600 Human Unigene: 35644 Mouse |
| 产品图片 |
Sample:
DU145(Human) Cell Lysate at 30 ug
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-NLRP14 (bs-19286R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kD
Observed band size: 125 kD
Sample:
Lane 1: Mouse Testis tissue lysates
Lane 2: Mouse Ovary tissue lysates
Lane 3: Rat Testis tissue lysates
Lane 4: Human SiHa cell lysates
Lane 5: Human JAR cell lysates
Primary: Anti-NLRP14 (bs-19286R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 125 kDa
Observed band size: 138 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
