| 产品编号 | bs-19327R |
| 英文名称 | NPEPL1 Rabbit pAb |
| 中文名称 | 氨肽酶样蛋白1抗体 |
| 别 名 | bA261P9.2; mKIAA1974; XELAEV_18046277mg; npepl1; wu:fi27h09; wu:fj47h12; zgc:112459; PEPL1_HUMAN; Aminopeptidase-like 1; 3.4.11.-; KIAA1974; PEPL1_MOUSE; aminopeptidase like 1 |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Cow, 56) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 55kDa |
| 检测分子量 | 55 |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NPEPL1: 371-470/494 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Aminopeptidases are widely distributed in eukaryotes and prokaryotes. These enzymes catalyze the removal of amino acids from the N-termini of proteins or peptide substrates. Aminopeptidases are involved in regulating hormone levels and are essential for digestive and intracellular protein metabolism. NPEPL1, also referred to as aminopeptidase-like 1, is a 523 amino acid protein that belongs to the peptidase M17 family and is ubiquitously expressed. NPEPL1 may be involved in the processing, catabolism and degradation of intracellular proteins by catalyzing the removal of unsubstituted N-terminal amino acids from various peptides. NPEPL1 contains several zinc binding sites and and is expressed as three isoforms due to alternative splicing events. Function: Probably catalyzes the removal of unsubstituted N-terminal amino acids from various peptides. Tissue Specificity: Ubiquitously expressed. Similarity: Belongs to the peptidase M17 family. SWISS: Q8NDH3 Gene ID: 79716 Database links: Entrez Gene: 79716 Human Entrez Gene: 228961 Mouse Entrez Gene: 734466 Xenopus laevis Entrez Gene: 613087 Xenopus tropicalis Entrez Gene: 550383 Zebrafish SwissProt: Q8NDH3 Human SwissProt: Q6NSR8 Mouse Unigene: 654868 Human Unigene: 295629 Mouse |
| 产品图片 |
Sample: lung (mouse) Lysate at 40 ug
Primary: Anti-NPEPL1(bs-19327R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
