| 产品编号 | bs-19627R |
| 英文名称 | SEPHS1 Rabbit pAb |
| 中文名称 | 硒磷酸化物合成酶1抗体 |
| 别 名 | MGC4980; SELD; SPS1_HUMAN; Selenide, water dikinase 1; Selenium donor protein 1; Selenophosphate synthetase 1; SEPHS1; SPS; SPS1. |
| 研究领域 | 细胞生物 免疫学 结合蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SEPHS1: 2-100/392 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes an enzyme that synthesizes selenophosphate from selenide and ATP. Selenophosphate is the selenium donor used to synthesize selenocysteine, which is co-translationally incorporated into selenoproteins at in-frame UGA codons. [provided by RefSeq, Sep 2010] Function: Synthesizes selenophosphate from selenide and ATP. Subunit: Homodimer (isoform 1, isoform 2, isoform 3 and isoform 4). Heterodimer of isoform 1 and isoform 3. Heterodimer of isoform 2 and isoform 4. Tissue Specificity: Isoform 1 and isoform 2 are gradually expressed during the cell cycle until G2/M phase and then decreased. Isoform 3 is gradually expressed during the cell cycle until S phase and then decreased. Similarity: Belongs to the selenophosphate synthase 1 family. Class II subfamily. SWISS: P49903 Gene ID: 22929 Database links: Entrez Gene: 22929 Human Entrez Gene: 426612 Chicken Entrez Gene: 109079 Mouse Entrez Gene: 379650 Xenopus laevis Entrez Gene: 448749 Xenopus tropicalis Entrez Gene: 324947 Zebrafish Omim: 600902 Human SwissProt: P49903 Human SwissProt: Q8BH69 Mouse SwissProt: Q6PF47 Xenopus laevis SwissProt: Q6GL12 Xenopus tropicalis SwissProt: Q7ZW38 Zebrafish Unigene: 124027 Human Unigene: 34329 Mouse Unigene: 100803 Rat |
| 产品图片 |
Protein: bone(mouse) lysate at 40ug;
Primary: rabbit Anti-SEPHS1(bs-19627R) at 1:300;
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 36 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
