| 产品编号 | bs-19653R |
| 英文名称 | SERCA3 Rabbit pAb |
| 中文名称 | 肌浆/内质网钙ATP酶3抗体 |
| 别 名 | SERCA; SERCA3; SERCA3b; XELAEV_18012809mg; atp2a3; AT2A3_CHICK; ChkSERCA3; SR Ca(2+)-ATPase 3; Calcium pump 3; 7.2.2.10; AT2A3_HUMAN; AT2A3_MOUSE; AT2A3_RAT; ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3; ATPase, Ca++ transporting, ubiquitous; sarcoplasmic/endoplasmic reticulum calcium ATPase 3 |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Chicken, Cow, Xenopus laevis, Xenopus tropic) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 114kDa |
| 检测分子量 | 110 |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SERCA3: 101-200/1043 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008] Function: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium. Transports calcium ions from the cytosol into the sarcoplasmic/endoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction. Subcellular Location: Nucleus membrane. Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Tissue Specificity: Found in most tissues. Most abundant in thymus, trachea, salivary gland, spleen, bone marrow, lymph node, peripheral leukocytes, pancreas and colon. Also detected in fetal tissues. Similarity: Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily. SWISS: Q93084 Gene ID: 489 Database links: Entrez Gene: 395707 Chicken Entrez Gene: 489 Human Entrez Gene: 53313 Mouse Entrez Gene: 495440 Xenopus laevis Entrez Gene: 779786 Xenopus tropicalis Omim: 601929 Human SwissProt: Q9YGL9 Chicken SwissProt: Q93084 Human SwissProt: Q64518 Mouse Unigene: 513870 Human Unigene: 6306 Mouse Unigene: 9920 Rat |
| 产品图片 |
Sample:
Lane 1: Human HepG2 cell lysates
Lane 2: Human Raji cell lysates
Lane 3: Human Jurkat cell lysates
Lane 4: Human HL-60 cell lysates
Primary: Anti-SERCA3 (bs-19653R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 114 kDa
Observed band size: 110 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
