| 产品编号 | bs-19699R |
| 英文名称 | QTRT1 Rabbit pAb |
| 中文名称 | 鸟嘌呤插入酶QTRT1抗体 |
| 别 名 | FP3235; Guanine insertion enzyme; qtrt1; Queuine tRNA ribosyltransferase 1; Queuine tRNA ribosyltransferase; Queuine tRNA-ribosyltransferase; TGT; TGT, 43 KD subunit; TGT, catalytic subunit; TGT_HUMAN; TGUT; tRNA guanine transglycosylase; tRNA-guanine transglycosylase. |
| 研究领域 | 细胞生物 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Guinea Pig, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human QTRT1: 201-300/403 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes the catalytic subunit of tRNA-guanine transglycosylase. tRNA-guanine transglycosylase is a heterodimeric enzyme complex that plays a critical role in tRNA modification by synthesizing the 7-deazaguanosine queuosine, which is found in tRNAs that code for asparagine, aspartic acid, histidine and tyrosine. A pseudogene of this gene is located on the long arm of chromosome X. [provided by RefSeq, Feb 2012] Function: Interacts with QTRTD1 to form an active queuine tRNA-ribosyltransferase. This enzyme exchanges queuine for the guanine at the wobble position of tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr), thereby forming the hypermodified nucleoside queuosine (Q) (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine). Subcellular Location: Cytoplasm. Mitochondrion. Nucleus. Weakly associates with mitochondria, possibly via QTRTD1. Similarity: Belongs to the queuine tRNA-ribosyltransferase family. SWISS: Q9BXR0 Gene ID: 81890 Database links: Entrez Gene: 81890 Human Entrez Gene: 60507 Mouse Entrez Gene: 393985 Zebrafish Omim: 609615 Human SwissProt: Q9BXR0 Human SwissProt: Q9JMA2 Mouse SwissProt: Q7SYK1 Zebrafish Unigene: 631638 Human Unigene: 436579 Mouse Unigene: 53858 Rat Unigene: 9747 Zebrafish |
| 产品图片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-QTRT1 (bs-19699R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 48 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
