| 产品编号 | bs-19727R |
| 英文名称 | Rad51L1 Rabbit pAb |
| 中文名称 | Rad51样蛋白1抗体 |
| 别 名 | DNA repair protein RAD51 homolog 2; hREC2; MGC34245; OTTHUMP00000212251; OTTHUMP00000212253; OTTHUMP00000212254; OTTHUMP00000212255; R51H2; RA51B_HUMAN; RAD51 homolog B(S. cerevisiae); RAD51 homolog B; RAD51 like 1; RAD51 like protein 1; RAD51-like protei |
| 研究领域 | 细胞生物 细胞凋亡 细胞周期蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Rabbit, ) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Rad51L1: 1-100/384 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are evolutionarily conserved proteins essential for DNA repair by homologous recombination. This protein has been shown to form a stable heterodimer with the family member RAD51C, which further interacts with the other family members, such as RAD51, XRCC2, and XRCC3. Overexpression of this gene was found to cause cell cycle G1 delay and cell apoptosis, which suggested a role of this protein in sensing DNA damage. At least three alternatively spliced transcript variants encoding distinct isoforms have been observed. Rearrangements between this locus and high mobility group AT-hook 2 (HMGA2, GeneID 8091) have been observed in uterine leiomyomata. [provided by RefSeq, Jul 2011] Function: Involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents. May promote the assembly of presynaptic RAD51 nucleoprotein filaments. The RAD51B-RAD51C dimer exhibits single-stranded DNA-dependent ATPase activity. The BCDX2 complex binds single-stranded DNA, single-stranded gaps in duplex DNA and specifically to nicks in duplex DNA. Subcellular Location: Nucleus. Tissue Specificity: Expressed in a wide range of tissues. DISEASE: Note=A chromosomal aberration involving RAD51L1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with HMGA1. Note=A chromosomal aberration involving RAD51L1 is found in uterine leiomyoma. Translocation t(12;14)(q15;q23-24) with HMGA2. Similarity: Belongs to the RecA family. RAD51 subfamily. SWISS: O15315 Gene ID: 5890 Database links: Entrez Gene: 5890 Human Entrez Gene: 467487 Chimpanzee Entrez Gene: 100053023 Horse Entrez Gene: 19363 Mouse Omim: 602948 Human SwissProt: O15315 Human SwissProt: O35719 Mouse Unigene: 172587 Human Unigene: 341756 Mouse |
| 产品图片 |
Sample:
U251(Human) Cell Lysate at 30 ug
Primary: Anti-Rad51L1 (bs-19727R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kD
Observed band size: 42 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
