| 产品编号 | bs-19989R |
| 英文名称 | PPP1R11 Rabbit pAb |
| 中文名称 | 蛋白磷酸酶PPP1R11抗体 |
| 别 名 | HCGV; IPP3; HCG-V; TCTE5; TCTEX5. |
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Specific References (2) | bs-19989R has been referenced in 2 publications.
111
[IF=2.923] Xianrong Xiong. et al. MicroRNA-34b-5p targets PPP1R11 to inhibit proliferation and promote apoptosis in cattleyak Sertoli cells by regulating specific signaling pathways. THERIOGENOLOGY. 2022 Dec;194:46 ICC, IHC, WB ; Bovine. 222
111
[IF=1.858] Xingyu Min. et al. Analysis of PPP1R11 expression in granulosa cells during developmental follicles of yak and its effects on cell function. REPROD DOMEST ANIM. 2022 Oct;: IHC, IF ; Yak. 222
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| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat, (predicted: Human, Mouse, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPP1R11: 21-100/126 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a specific inhibitor of protein phosphatase-1 (PP1) with a differential sensitivity toward the metal-independent and metal-dependent forms of PP1. The gene is located within the major histocompatibility complex class I region on chromosome 6. [provided by RefSeq, Jul 2008] Function: Inhibitor of protein phosphatase 1. Subcellular Location: Cytoplasmic. Tissue Specificity: Widely expressed. SWISS: O15091 Gene ID: 9692 Database links: Entrez Gene: 6992 Human Entrez Gene: 76497 Mouse Omim: 606670 Human SwissProt: O60927 Human SwissProt: Q8K1L5 Mouse Unigene: 82887 Human Unigene: 46176 Mouse Unigene: 63970 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPP1R11) Polyclonal Antibody, Unconjugated (bs-19989R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
