| 产品编号 | bs-21038R |
| 英文名称 | ROD1/PTBP3 Rabbit pAb |
| 中文名称 | 调节分化蛋白ROD1抗体 |
| 别 名 | DKFZp781I1117; Fission yeast differentiation regulator; OTTHUMP00000021931; OTTHUMP00000021932; Polypyrimidine tract binding protein 3; regulator of differentiation 1; Regulator of differentiation(in S. pombe) 1; Regulator of differentiation 1; ROD 1; Rod1; ROD1 regulator of differentiation 1; ROD1_HUMAN. |
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Specific References (1) | bs-21038R has been referenced in 1 publications.
111
[IF=2.095] Zhou W et al. MiR-384 inhibits proliferation and migration of trophoblast cells via targeting PTBP3. Pregnancy Hypertens
. 2020 Jul;21:132-138. WB ; Human. 222
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| 研究领域 | 细胞生物 结合蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, Rat, (predicted: Human, Dog, Pig, Cow, Horse, Rabbit, Zebrafish, Sheep, Danio) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ROD1/PTBP3: 201-300/552 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene binds RNA and is a regulator of cell differentiation. The encoded protein preferentially binds to poly(G) and poly(U) sequences in vitro. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011] Function: RNA-binding protein that mediates pre-mRNA alternative splicing regulation. Plays a role in the regulation of cell proliferation, differentiation and migration. Positive regulator of EPO-dependent erythropoiesis. Participates in cell differentiation regulation by repressing tissue-specific exons. Promotes FAS exon 6 skipping. Binds RNA, preferentially to both poly(G) and poly(U). Subcellular Location: Contains 4 RRM (RNA recognition motif) domains. Tissue Specificity: Expressed in several hematopoietic cell lines examined. Similarity: Contains 4 RRM (RNA recognition motif) domains. SWISS: O95758 Gene ID: 9991 Database links: Entrez Gene: 9991 Human Entrez Gene: 230257 Mouse Omim: 607527 Human SwissProt: O95758 Human SwissProt: Q8BHD7 Mouse Unigene: 269988 Human Unigene: 331640 Mouse Unigene: 162818 Rat |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-ROD1/PTBP3 (bs-21038R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 60 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
