| 产品编号 | bs-21055R |
| 英文名称 | PPEF1 Rabbit pAb |
| 中文名称 | PPEF1蛋白抗体 |
| 别 名 | PP 7; PP7; PPE1_HUMAN; PPEF 1; PPEF; PPEF-1; Ppef1; PPP 7C; PPP7C; PPP7CA; Protein phosphatase 7, catalytic subunit, alpha isozyme; Protein phosphatase EF hand calcium binding domain 1; Protein phosphatase serine/threonine type with EF hands; Protein phosphatase with EF calcium binding domain; Protein phosphatase with EF calcium-binding domain; Protein phosphatase with EF hand calcium binding domain 1; Serine/threonine protein phosphatase 7; Serine/threonine protein phosphatase with EF hands 1; Serine/threonine-protein phosphatase 7; Serine/threonine-protein phosphatase with EF-hands 1. |
| 研究领域 | 细胞生物 神经生物学 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 75 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PPEF1: 301-400/653 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein has been suggested to play a role in specific sensory neuron function and/or development. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. Several alternatively spliced transcript variants, each encoding a distinct isoform, have been described. [provided by RefSeq, Jul 2008] Function: May have a role in the recovery or adaptation response of photoreceptors. May have a role in development. Tissue Specificity: Detected in retina and retinal derived Y-79 retinoblastoma cells. Also found in fetal brain. Similarity: Belongs to the PPP phosphatase family. Contains 3 EF-hand domains. Contains 1 IQ domain. SWISS: O14829 Gene ID: 5475 Database links: Entrez Gene: 5475 Human Omim: 300109 Human SwissProt: O14829 Human Unigene: 211589 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PPEF1) Polyclonal Antibody, Unconjugated (bs-21055R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
