| 产品编号 | bs-4491R |
| 英文名称 | PLA2G4E Rabbit pAb |
| 中文名称 | 胞浆型磷脂酶A2E抗体 |
| 别 名 | 2310026J01Rik; C230096D22; Pla2epsilon; RGD1310595; PA24E_HUMAN; PLA2G4E; cPLA2-epsilon; Calcium-dependent N-acyltransferase; Phospholipase A2 group IVE; 3.1.1.4; PA24E_MOUSE; |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Cow, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 97 kDa |
| 检测分子量 | 100 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PLA2G4E: 451-550/856 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PLA2G4E is a calcium-dependent phospholipase A2 that selectively hydrolyzes glycerophospholipids in the sn-2 position. Subcellular Location: Cytoplasm, cytosol. Lysosome membrane, Peripheral membrane protein. Similarity: Contains 1 C2 domain. Contains 1 PLA2c domain. SWISS: Q3MJ16 Gene ID: 123745 Database links: Entrez Gene: 123745 Human Entrez Gene: 329502 Mouse SwissProt: Q3MJ16 Human SwissProt: Q50L42 Mouse |
| 产品图片 |
Sample:
Lane 1: Mouse Muscle tissue lysates
Lane 2: Rat Muscle tissue lysates
Primary: Anti-PLA2G4E (bs-4491R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 100 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
