产品编号 | bs-12735R |
英文名称 | PLS1 Rabbit pAb |
中文名称 | 肠道特异性凝血酶PLS1抗体 |
别 名 | Fimbrin; I Fimbrin; I Plastin; Intestine specific plastin; Plastin 1(I isoform); Plastin 1; Plastin1. |
研究领域 | 细胞生物 信号转导 结合蛋白 细胞骨架 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human, Mouse, Rat, Dog, Cow, Horse, Rabbit, Sheep, ) |
产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 70 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PLS1: 41-140/329 |
亚 型 | |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010] Function: Actin-bundling protein in the absence of calcium. Subcellular Location: Cytoplasm. Tissue Specificity: In small intestine, colon, and kidney; relatively lower levels of expression are detected in the lung and stomach. Post-translational modifications: Phosphorylated. Similarity: Contains 2 actin-binding domains. Contains 4 CH (calponin-homology) domains. Contains 2 EF-hand domains. SWISS: Q14651 Gene ID: 5357 Database links: Entrez Gene: 5357 Human Entrez Gene: 102502 Mouse Omim: 602734 Human SwissProt: Q14651 Human SwissProt: Q3V0K9 Mouse Unigene: 203637 Human |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |