产品编号 | bs-12740R |
英文名称 | PMM1 Rabbit pAb |
中文名称 | 磷酸甘露糖变位酶1抗体 |
别 名 | Brain glucose-1, 6-bisphosphatase; phosphomannomutase 1; PMM 1; pmm1; PMM1_HUMAN; PMMH 22; PMMH-22; PMMH22; Sec53. |
研究领域 | 肿瘤 细胞生物 神经生物学 信号转导 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 30 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PMM1: 31-130/262 |
亚 型 | |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
Phosphomannomutase catalyzes the conversion between D-mannose 6-phosphate and D-mannose 1-phosphate which is a substrate for GDP-mannose synthesis. GDP-mannose is used for synthesis of dolichol-phosphate-mannose, which is essential for N-linked glycosylation and thus the secretion of several glycoproteins as well as for the synthesis of glycosyl-phosphatidyl-inositol (GPI) anchored proteins. [provided by RefSeq, Jul 2008] Function: Involved in the synthesis of the GDP-mannose and dolichol-phosphate-mannose required for a number of critical mannosyl transfer reactions. In addition, may be responsible for the degradation of glucose-1,6-bisphosphate in ischemic brain. Subcellular Location: Cytoplasm. Tissue Specificity: Strong expression in liver, heart, brain, and pancreas; lower expression in skeletal muscle. Similarity: Belongs to the eukaryotic PMM family. SWISS: Q92871 Gene ID: 5372 Database links: Entrez Gene: 5372 Human Entrez Gene: 29858 Mouse Omim: 601786 Human SwissProt: Q92871 Human SwissProt: O35621 Mouse Unigene: 75835 Human Unigene: 18939 Mouse |
产品图片 |
Sample: 293T(Human) Cell Lysate at 40 ug
Primary: Anti-PMMI (bs-12740R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |