| 产品编号 | bs-12751R |
| 英文名称 | POFUT2 Rabbit pAb |
| 中文名称 | 蛋白O岩藻糖基转移酶2抗体 |
| 别 名 | Dmel_CG14789; CG14789; Dmel\CG14789; EG:BACN32G11.6; O-FUT2; O-fut; OFut2; C21orf80; FUT13; 2310011G23Rik; OFUT2_DROME; Peptide-O-fucosyltransferase 2 (O-FucT-2); 2.4.1.221; OFUT2_HUMAN; POFUT2; KIAA0958; OFUT2_MOUSE; |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, (predicted: Mouse, Rat, Chicken, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25, 50 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human POFUT2: 61-160/429 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Fucose is typically found as a terminal modification of branched chain glycoconjugates, but it also exists in direct O-linkage to serine or threonine residues within cystine knot motifs in epidermal growth factor (EGF; MIM 131530)-like repeats or thrombospondin (THBS; see MIM 188060) type-1 repeats. POFUT2 is an O-fucosyltransferase that use THBS type-1 repeats as substrates (Luo et al., 2006 [PubMed 16464857]).[supplied by OMIM, Mar 2008] Function: Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in thrombospondin type 1 repeats. Subcellular Location: Endoplasmic reticulum. Tissue Specificity: Isoform A is expressed in fetal liver and peripheral blood lymphocytes. Isoform B is expressed in spleen, lung, testis, bone marrow, thymus, pancreas, prostate, fetal brain, fetal liver and fetal kidney. Isoform C is expressed in brain, heart, spleen, liver, lung, stomach, testis, placenta, skin, thymus, pancreas, mammary gland, prostate, fetal brain, fetal liver and fetal heart. Similarity: Belongs to the glycosyltransferase 68 family. SWISS: Q9Y2G5 Gene ID: 23275 Database links: Entrez Gene: 31098 Fruit fly (Drosophila melanogaster) Entrez Gene: 23275 Human Entrez Gene: 80294 Mouse Omim: 610249 Human SwissProt: Q9W589 Fruit fly (Drosophila melanogaster) SwissProt: Q9Y2G5 Human SwissProt: Q8VHI3 Mouse Unigene: 4643 Fruit fly (Drosophila melanogaster) Unigene: 592164 Human Unigene: 203556 Mouse Unigene: 11702 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (POFUT2) Polyclonal Antibody, Unconjugated (bs-12751R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
