| 产品编号 | bs-12795R |
| 英文名称 | ZNF185 Rabbit pAb |
| 中文名称 | 锌指蛋白185抗体 |
| 别 名 | LIM domain protein ZNF185; P1 A; P1-A; Zinc finger protein 185(LIM domain); Zinc finger protein 185; ZN185_HUMAN; ZNF185. |
| 研究领域 | 肿瘤 细胞生物 锌指蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, Mouse, Rat, Cow, Horse, Sheep, ) |
| 产品应用 | ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 74 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZNF185: 101-200/689 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Zinc-finger proteins bind nucleic acids and play important roles in various cellular functions, including cell proliferation, differentiation, and apoptosis. This gene encodes a LIM-domain zinc finger protein. The LIM domain is composed of two contiguous zinc finger domains, separated by a two-amino acid residue hydrophobic linker. The LIM domain mediates protein:protein interactions. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, May 2010] Function: May be involved in the regulation of cellular proliferation and/or differentiation. Subcellular Location: Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Tissue Specificity: Expressed in placenta, pancreas and kidney. Also expressed in prostate, testis, ovary and blood. Similarity: Contains 1 LIM zinc-binding domain. SWISS: O15231 Gene ID: 7739 Database links: Entrez Gene: 7739 Human Entrez Gene: 22673 Mouse Omim: 300381 Human SwissProt: O15231 Human SwissProt: Q62394 Mouse Unigene: 16622 Human Unigene: 1161 Mouse |
| 产品图片 |
Blank control（black line）:Hela.
Primary Antibody (green line): Rabbit Anti-ZNF185 antibody (bs-12795R)
Dilution:2ug/Test;
Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
