| 产品编号 | bs-8635R |
| 英文名称 | Phostensin Rabbit pAb |
| 中文名称 | 蛋白磷酸酶1调节亚单位18抗体 |
| 别 名 | HKMT1098; KIAA1949; PPP1R18; Protein phosphatase 1 F actin cytoskeleton targeting subunit; Protein phosphatase 1 regulatory subunit 18. |
| 研究领域 | 细胞生物 信号转导 结合蛋白 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, ) |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 68 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Phostensin: 101-200/613 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Protein phosphatase-1 (PP1; see MIM 176875) interacts with regulatory subunits that target the enzyme to different cellular locations and change its activity toward specific substrates. Phostensin is a regulatory subunit that targets PP1 to F-actin (see MIM 102610) cytoskeleton (Kao et al., 2007 [PubMed 17374523]).[supplied by OMIM, Mar 2008] Subunit: Interacts with Protein phosphatase 1 (PP1). Subcellular Location: Cytoplasm; cytoskeleton. SWISS: Q6NYC8 Gene ID: 170954 Database links: Entrez Gene: 100051083 Horse Entrez Gene: 170954 Human Omim: 610990 Human SwissProt: Q6NYC8 Human Unigene: 101150 Human |
| 产品图片 |
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti- Phostensin (bs-8635R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 68 kD
Observed band size: 63 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
