| 产品编号 | bs-20188R |
| 英文名称 | ALPPL2 Rabbit pAb |
| 中文名称 | 碱性磷酸酶(类胎盘型)抗体 |
| 别 名 | Akp5; Alkaline phosphatase; Alkaline phosphatase Nagao isozyme; Alkaline phosphatase placental like; ALP 1; ALP-1; ALP1; ALPG; ALPPL 2; ALPPL; C77216; D1Ertd816e; GCAP; Germ cell alkaline phosphatase; Nagao isozyme; Placental alkaline phosphatase-like; Pl 类胎盘碱性磷酸酶; 碱性磷酸酶类胎盘; |
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Specific References (1) | bs-20188R has been referenced in 1 publications.
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[IF=10.383] Peiyang Gu. et al. Tailorable 3DP Flexible Scaffolds with Porosification of Filaments Facilitate Cell Ingrowth and Biomineralized Deposition. ACS APPL MATER INTER. 2022;XXXX(XXX):XXX-XXX WB ; Mouse. 222
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| 研究领域 | 肿瘤 激酶和磷酸酶 新陈代谢 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 53 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ALPPL2: 301-400/532 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The intestinal alkaline phosphatase gene encodes a digestive brush-border enzyme. This enzyme is upregulated during small intestinal epithelial cell differentiation. [provided by RefSeq, Jul 2008] Subunit: Homodimer. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor. Tissue Specificity: Trace amounts in the testis and thymus, and in elevated amounts in germ cell tumors. Similarity: Belongs to the alkaline phosphatase family. SWISS: P10696 Gene ID: 251 Database links: Entrez Gene: 251 Human Omim: 171810 Human SwissProt: P10696 Human Unigene: 333509 Human
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| 产品图片 |
Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ALPPL2 Polyclonal Antibody, Unconjugated(bs-20188R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Blank control (blue line): Hep G2(fixed with 70% ethanol Overnight at 4℃).
Primary Antibody (green line):Rabbit Anti-ALPPL2 antibody (bs-20188R),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
