| 产品编号 | bsm-30002M |
| 英文名称 | human CD2 Mouse mAb |
| 中文名称 | 小鼠抗人CD2单克隆抗体 |
| 别 名 | CD2_HUMAN; T-cell surface antigen CD2; T cell surface antigen CD2; SRBC; Erythrocyte receptor; LFA-2; LFA2; LFA3 receptor; LFA-3 receptor; Rosette receptor; T-cell surface antigen T11/Leu-5; CD 2; CD2 antigen; CD2 molecule; |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 2F4 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt= ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 37 kDa |
| 检测分子量 | 50 |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CD2 |
| 亚 型 | Mouse IgG1, k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a surface antigen found on all peripheral blood T-cells. The encoded protein interacts with LFA3 (CD58) on antigen presenting cells to optimize immune recognition. A locus control region (LCR) has been found in the 3' flanking sequence of this gene. [provided by RefSeq, Jun 2016] SWISS: P06729 Gene ID: 914 Database links: Entrez Gene: 914 Human Entrez Gene: 12481 Mouse Omim: 186990 Human SwissProt: P06729 Human SwissProt: P08920 Mouse Unigene: 523500 Human Unigene: 22842 Mouse Unigene: 10328 Rat |
| 产品图片 |
Sample:
Lane 1: Human Jurkat cell lysates
Primary: Anti-human CD2 (bsm-30002M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 37 kDa
Observed band size: 50 kDa
scatter diagram showing PBMC stained with CD2. The cells were incubated with the antibody (bsm-30002M,1μg/Test) for 30 min at 22°C.The secondary antibody used for 40 min at room temperature. Acquisition of >10,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
