| 产品编号 | bs-20263R |
| 英文名称 | Pescadillo Rabbit pAb |
| 中文名称 | Pescadillo抗体 |
| 别 名 | FLJ43790; Homo sapiens pescadillo mRNA complete cds; PES 1; PES; pes1; PESC_HUMAN; Pescadillo(zebrafish) homolog 1 containing BRCT domain; Pescadillo homolog 1; Pescadillo homolog 1 containing BRCT domain(zebrafish); Pescadillo homolog; Pescadillo ribosom |
| 研究领域 | 肿瘤 细胞生物 免疫学 信号转导 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, (predicted: Mouse, Rat, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 68 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Pescadillo: 21-120/588 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pescadillo may play a crucial role in cell proliferation and may be necessary for oncogenic transformation and tumor progression. The protein is abnormally elevated in malignant tumors of astrocytic origin. It is a strongly conserved gene containing a BRCT domain that is essential for the activity of this gene product. Function: Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome. Subunit: Interacts with IRS1 and UBTF. May interact with MAP1B (By similarity). Component of the PeBoW complex, composed of BOP1, PES1 and WDR12. Within the PeBoW complex BOP1 interacts directly with PES1 and WDR12. The PeBoW complex also associates with the 66S pre-ribosome. Subcellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm. Chromosome. Note=Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli. Tissue Specificity: Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin. Post-translational modifications: Sumoylated. Similarity: Belongs to the pescadillo family. Contains 1 BRCT domain. SWISS: O00541 Gene ID: 23481 Database links: Entrez Gene: 23481 Human Entrez Gene: 64934 Mouse Omim: 605819 Human SwissProt: O00541 Human SwissProt: Q5SQ20 Mouse SwissProt: Q9EQ61 Mouse Unigene: 517543 Human Unigene: 28659 Mouse Unigene: 9521 Rat |
| 产品图片 |
Sample: Hela(human)cell Lysate at 40 ug
Primary: Anti-Pescadillo(bs-20263R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 68kD
Observed band size: 63kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
