| 产品编号 | bs-20098R |
| 英文名称 | PRPF4 Rabbit pAb |
| 中文名称 | PRPF4蛋白抗体 |
| 别 名 | HPRP4; HPRP4P; PRP4; PRP4 pre mRNA processing factor 4 homolog(yeast); PRP4/STK/WD splicing factor; Prp4p; U4/U6 small nuclear ribonucleoprotein Prp4; WD splicing factor Prp4; PRP4_HUMAN. |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human, Mouse, (predicted: Rat, Dog, Pig, Cow, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRPF4: 431-522/522 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pre-mRNA splicing occurs in two sequential transesterification steps, and the protein encoded by this gene is thought to be involved in pre-mRNA splicing and in signal transduction. This protein belongs to a kinase family that includes serine/arginine-rich protein-specific kinases and cyclin-dependent kinases (CDKs). This protein is regarded as a CDK-like kinase (Clk) with homology to mitogen-activated protein kinases (MAPKs). [provided by RefSeq, Jul 2008] Function: Involved in pre-mRNA splicing. Subunit: Interacts directly with PRPF18, PPIH and PRPF3. Part of a heteromeric complex containing PPIH, PRPF3 and PRPF4 that is stable in the absence of RNA. This complex interacts with the U4/U5/U6 tri-snRNP complex. Subcellular Location: Nucleus speckle. Note=Colocalizes with spliceosomal snRNPs. Similarity: Contains 7 WD repeats. SWISS: Q13523 Gene ID: 8899 Database links: Entrez Gene: 8899 Human Entrez Gene: 19134 Mouse Omim: 602338 Human SwissProt: Q13523 Human SwissProt: Q61136 Mouse Unigene: 159014 Human Unigene: 10027 Mouse Unigene: 482253 Mouse Unigene: 32654 Rat |
| 产品图片 |
Sample:
Raji (Human) Cell Lysate at 40 ug
HepG2 (Human) Cell Lysate at 40 ug
Hela (Human) Cell Lysate at 40 ug
Primary: Anti-PRPF4(bs-20098R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 58kD
Observed band size: 58kD
Sample:
bone (mouse) Lysate at 40 ug
lymph node (mouse) Lysate at 40 ug
Primary: Anti-PRPF4(bs-200098R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 58 kD
Observed band size: 58 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
