| 产品编号 | bs-20757R |
| 英文名称 | BRN4 Rabbit pAb |
| 中文名称 | 脑转录因子4蛋白抗体 |
| 别 名 | BRAIN-4; BRN-4; BRN4; DFN3; DFNX2; OCT-9; OTF-9; OTF9; Slf; RHS2; PO3F4_HUMAN; POU3F4; Brain-specific homeobox/POU domain protein 4 (Brain-4 | Brn-4); Octamer-binding protein 9 (Oct-9); Octamer-binding transcription factor 9 (OTF-9); PO3F4_MOUSE; PO3F4_RA |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | (predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 51 kDa |
| 检测分子量 | 51 |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BRN4: 1-100/361 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain.Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) . A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. Function: Probable transcription factor which exert its primary action widely during early neural development and in a very limited set of neurons in the mature brain. Subunit: Homodimer. Heterodimer with a RAR molecule. Binds DNA preferentially as a RAR/RXR heterodimer. Subcellular Location: Nucleus. Tissue Specificity: Brain specific. DISEASE: Defects in POU3F4 are a cause of deafness X-linked type 2 (DFNX2) [MIM:304400]. A mixed type of deafness characterized by both conductive hearing loss resulting from stapes (perilymphatic gusher) fixation and progressive sensorineural deafness. Similarity: Belongs to the POU transcription factor family. Class-3 subfamily. Contains 1 homeobox DNA-binding domain. Contains 1 POU-specific domain. SWISS: P49335 Gene ID: 5456 Database links: Entrez Gene: 5456 Human Entrez Gene: 18994 Mouse Omim: 300039 Human SwissProt: P49335 Human SwissProt: P62515 Mouse Unigene: 2229 Human Unigene: 405149 Mouse Unigene: 56946 Mouse Unigene: 33030 Rat
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| 产品图片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Cerebellum (Mouse) Lysate at 40 ug
Primary: Anti-BRN4 (bs-20757R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51 kD
Observed band size: 51 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
