| 产品编号 | bs-23111R |
| 英文名称 | Ly-6G Rabbit pAb |
| 中文名称 | 淋巴细胞抗原6G抗体 |
| 别 名 | Gr-1; Gr1; Ly 6G; Ly-6G.1; Ly6G; lymphocyte antigen 6 complex locus G; Lymphocyte antigen 6G; LY6G_MOUSE. |
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Specific References (1) | bs-23111R has been referenced in 1 publications.
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[IF=3.269] Meihua Ji. et al. Oxycodone protects cardiac microvascular endothelial cells against ischemia/reperfusion injury by binding to Sigma-1 Receptor. BIOENGINEERED. Bioengineered. 2022;13(4):9628-9644 IF ; Rat. 222
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| 研究领域 | 细胞生物 免疫学 细胞表面分子 淋巴细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse, |
| 产品应用 | ELISA=1:5000-10000 Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 14 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Ly-6G: 4-50/134 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Ly6G is a GPI-anchored protein, that is also known as the myeloid differentiation antigen Gr1. The antigen is transiently expressed on monocytes in the bone marrow. The level of antigen expression in the bone marrow directly correlates with granulocyte differentiation and maturation. Ly6G is expressed predominantly on neutrophils, also in a subset of eosinophils, differentiating pre-monocytes, and plasmacytoid dendritic cells. Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor (By similarity). Tissue Specificity: Expressed in bone marrow. Similarity: Contains 1 UPAR/Ly6 domain. SWISS: P35461 Gene ID: 546644 Database links: Entrez Gene: 546644 Mouse SwissProt: P35461 Mouse Ly6G属于是Ly-6家族成员,主要表达于T细胞、单核细胞，中性白细胞等，在抑制抗肿瘤和T细胞的应答方面起着重要作用。只要定位于细胞膜、细胞浆。 |
| 产品图片 |
Blank control: sp2/0.
Primary Antibody (green line): Rabbit Anti-Ly-6G antibody (bs-23111R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
