| 产品编号 | bsm-33272M |
| 英文名称 | Alpha-actinin-1 Mouse mAb |
| 中文名称 | α-辅肌动蛋白1单克隆抗体 |
| 别 名 | ACTN 1; ACTN1_HUMAN; alpha Actinin 1; alpha actinin cytoskeletal isoform; alpha-actinin cytoskeletal isoform; alpha-actinin-1; FLJ40884; FLJ54432; Actinin alpha 1; F-Actin Cross-Linking Protein; Non-Muscle alpha-Actinin-1; Actinin, alpha 1; Actinin 1 Smooth Muscle; BDPLT15; |
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Specific References (1) | bsm-33272M has been referenced in 1 publications.
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[IF=5.5] Chen, Yejia. et al. Complement C5a induces the generation of neutrophil extracellular traps by inhibiting mitochondrial STAT3 to promote the development of arterial thrombosis. THROMB J. 2022 Dec;20(1):1-14 WB ; Human. 222
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| 研究领域 | 细胞生物 发育生物学 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 6A10 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:500-1000 ICC=1:100-500 IF=1:500-1000 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 103 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Alpha-actinin-1 protein 1-205/894 (C-6x His-Tag) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Subunit: Homodimer; antiparallel. Interacts with DDN, MYOZ2, PDLIM2, TTID and LPP. Interacts with PSD. Interacts with MICALL2 (By similarity). Interacts with DNM2 and CTTN (By similarity). Subcellular Location: Cytoplasm > cytoskeleton. Cytoplasm > myofibril > sarcomere > Z line. Cell membrane. Cell junction. Cell projection › ruffle. Similarity: Belongs to the alpha-actinin family. Contains 1 actin-binding domain. Contains 2 CH (calponin-homology) domains. Contains 2 EF-hand domains. Contains 4 spectrin repeats. SWISS: P12814 Gene ID: 87 Database links:
Entrez Gene: 87 Human Entrez Gene: 109711 Mouse Omim: 102575 Human SwissProt: P12814 Human SwissProt: Q7TPR4 Mouse Unigene: 509765 Human Unigene: 253564 Mouse Unigene: 403477 Mouse Unigene: 6401 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
