| 产品编号 | bsm-33063M |
| 英文名称 | CDX2 Mouse mAb |
| 中文名称 | 尾型同源盒转录因子2单克隆抗体 |
| 别 名 | cau-dal-related homeobox transcription factor 2; Caudal type homeo box 2; Caudal type homeobox protein 2; caudal type homeobox transcription factor 2; CDX 2; CDX 3; CDX3; Homeobox protein CDX-2; Homeobox protein CDX2; CDX2_HUMAN. |
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Specific References (1) | bsm-33063M has been referenced in 1 publications.
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[IF=4.175] Li Liang. et al. Identification of key genes involved in tumor immune cell infiltration and cetuximab resistance in colorectal cancer. Cancer Cell Int. 2021 Dec;21(1):1-22 IHC ;
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| 研究领域 | 肿瘤 发育生物学 染色质和核信号 转录调节因子 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 5G8 |
| 交叉反应 | Rat, (predicted: Human, Mouse, ) |
| 产品应用 | WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CDX2 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Homeobox protein CDX-2 is a protein that in humans is encoded by the CDX2 gene. This gene is a member of the caudal-related homeobox transcription factor family that is expressed in the nuclei of intestinal epithelial cells. CDX2 is also used in diagnostic surgical pathology as a marker for gastrointestinal differentiation, especially colorectal. Function: Involved in the transcriptional regulation of multiple genes expressed in the intestinal epithelium. Important in broad range of functions from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine. Subcellular Location: Nucleus. Post-translational modifications: Phosphorylation of Ser-60 mediates the transactivation capacity. Similarity: Belongs to the Caudal homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q99626 Gene ID: 1045 Database links: Entrez Gene: 1045 Human Entrez Gene: 12591 Mouse Omim: 600297 Human SwissProt: Q99626 Human SwissProt: P43241 Mouse Unigene: 174249 Human Unigene: 20358 Mouse Unigene: 64495 Rat 核转录因子抗体 CDX2表达主要用于消化系统肿瘤及癌前病变的研究。用于与肿瘤的增值、分化、大小、临床分期及分化程度等关系方面的研究。CDX2是一种肠特异的转录因子，CDX2能够调节肠上皮细胞的增殖和分化，定位于肠上皮的绒毛和隐窝表面。据报道95%以上的浸润性结/直肠癌中均可检出CDX2阳性表达，也可在胃癌和卵巢黏液性腺癌中表达。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CDX2) Monoclonal Antibody, Unconjugated (ascites of bsm-33063M 5G8) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
