| 产品编号 | bsm-33111M |
| 英文名称 | FUT4 Mouse mAb |
| 中文名称 | α-(1,3)-岩藻糖基转移酶 4单克隆抗体 |
| 别 名 | FUT4_HUMAN; alpha-(1, 3)-fucosyltransferase 4; 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase; EC:2.4.1.152; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV(Fuc-TIV; FucT-IV); Galactoside 3-L-fucosyltransferase; ELFT; FCT3A; LeX; CD15; FUTIV; SSEA-1; SSEA1; FUC-TIV; |
| 研究领域 | 肿瘤 细胞生物 免疫学 细胞膜受体 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 8B9 |
| 交叉反应 | Human, |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 58 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human FUT4 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq]. Function: May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens. Subcellular Location: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Note=Membrane-bound form in trans cisternae of Golgi. Tissue Specificity: Highest expression in stomach and colon. It is also expressed in the lung, testis, uterus, small intestine and to a lesser extent in spleen, and ovary. Present in trace amounts in brain, thymus, heart, smooth muscle, kidney and bone marrow. Not found in liver, salivary gland and pancreas. Similarity: Belongs to the glycosyltransferase 10 family. SWISS: P22083 Gene ID: 2526 Database links: Entrez Gene: 10690 Human Entrez Gene: 2526 Human Entrez Gene: 2527 Human Entrez Gene: 2528 Human Entrez Gene: 2529 Human Omim: 104230 Human SwissProt: P22083 Human SwissProt: P51993 Human SwissProt: Q11128 Human SwissProt: Q11130 Human SwissProt: Q9Y231 Human Unigene: 390420 Human Unigene: 457 Human Unigene: 49117 Human Unigene: 572064 Human Unigene: 623098 Human Unigene: 631843 Human Unigene: 631846 Human Unigene: 705615 Human FUT4，也称为 ELFT 和 FCT3A，属于糖基转移酶 10 家族。FUT4 可催化参与 Lewis X/SSEA-1 和 VIM-2 抗原表达的 alpha-1,3 糖苷键。FUT4 是一种抗原表位，定义为 Lewis X 碳水化合物结构，在鼠胚胎癌细胞 (EC)、鼠 ES 和 iPS 细胞以及鼠和人类生殖细胞上表达。它被广泛用作小鼠未分化 ES 和 iPS 细胞的阳性表面标志物和人未分化 ES 和 iPS 细胞的阴性表面标志物。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human esophageal); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Monoclonal Antibody, Unconjugated (ascites of bsm-33111M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FUT4) Monoclonal Antibody, Unconjugated (ascites of bsm-33111M) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
