| 产品编号 | bsm-33202M |
| 英文名称 | Acetyl Lysine Mouse mAb |
| 中文名称 | 乙酰基赖氨酸/乙酰化赖氨酸抗体 |
| 别 名 | Acetylated Lysine; Acetyllysine; Ac-lysine; Ac lysine; Ac-K; Ac K; Ac-Lys; Ac Lys; acetyl-Lysine; acetyl Lys; Ac-Lysine; 赖氨酸乙酰化; 乙酰化修饰赖氨酸；乙酰赖氨酸； |
| 产品类型 | 小分子抗体 |
| 研究领域 | 细胞生物 免疫学 表观遗传学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 10C12 |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | kDa |
| 检测分子量 | |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | BSA conjugated Acetylated Lysine |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | |
| 产品图片 |
4% Paraformaldehyde-fixed Hela（Treated Sodium Butyrate (5mM, 24h)） (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Acetyl Lysine) monoclonal Antibody, unconjugated (bsm-33202M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-M
The Hela (Treated Sodium Butyrate (5mM, 24h))(H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
