| 产品编号 | bsm-10895M |
| 英文名称 | TIMP-1 Mouse mAb |
| 中文名称 | 金属蛋白酶组织抑制因子-1单克隆抗体 |
| 别 名 | Clgi; Collagenase inhibitor; EPA; EPO; Erythroid Potentiating Activity; Fibroblast collagenase inhibitor; FLJ90373; HC; Human Collagenase Inhibitor; Metalloproteinase inhibitor 1; Metalloproteinase inhibitor 1 precursor; OTTHUMP00000023214; TIMP 1; TIMP; TIMP metallopeptidase inhibitor 1; TIMP1 protein; Tissue Inhibitor of Metalloproteinase 1; Tissue inhibitor of metalloproteinases; Ttissue inhibitor of metalloproteinase 1 erythroid potentiating activity collagenase inhibitor. |
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Specific References (2) | bsm-10895M has been referenced in 2 publications.
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[IF=5.714] Yipeng Wan. et al. Ursolic acid alleviates Kupffer cells pyroptosis in liver fibrosis by the NOX2/NLRP3 inflammasome signaling pathway. INT IMMUNOPHARMACOL. 2022 Dec;113:109321 WB ; Mouse. 222
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[IF=3.801] M Li. et al. Histology‐based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers. 2021 Mar 14 IF ; Rat. 222
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| 研究领域 | 肿瘤 细胞生物 神经生物学 细胞凋亡 生长因子和激素 激酶和磷酸酶 合成与降解 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4C2 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:200-800 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human TIMP-1 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq]. Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Subcellular Location: Secreted. Post-translational modifications: The activity of TIMP1 is dependent on the presence of disulfide bonds. Similarity: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. SWISS: P01033 Gene ID: 7076 Database links: Entrez Gene: 7076 Human Entrez Gene: 21857 Mouse Omim: 305370 Human SwissProt: P01033 Human SwissProt: P12032 Mouse Unigene: 522632 Human Unigene: 8245 Mouse |
| 产品图片 |
4% Paraformaldehyde-fixed Hela (treated with 500nM BFA for 2 hours) (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (TIMP1) monoclonal Antibody, unconjugated (bsm-10895M) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
The Hela (treated with 500nM BFA for 2 hours) (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by secondary antibody incubation for 40 min at room temperature. Primary Antibody (green):Mouse Anti-TIMP1 antibody (bsm-10895M): 1 μg/10^6 cells; Isotype Control (orange): Mouse IgG (bs-0296P). Blank control (black): PBS. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
