| 产品编号 | bs-23373R |
| 英文名称 | Glutaredoxin 1 Rabbit pAb |
| 中文名称 | 谷氧还蛋白/巯基转移酶抗体 |
| 别 名 | GLRXL; GRX; GRX1; D13Wsu156e; Glrx1; TTase; TTF; GLRX1_BOVIN; GLRX; Thioltransferase (TTase); GLRX1_HUMAN; Thioltransferase-1 (TTase-1); GLRX1_MOUSE; GLRX1_PIG; GLRX1_RABIT; GLRX1_RAT; glutaredoxin; glutaredoxin (thioltransferase); thioltransferase; Glutaredoxin-1 |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, (predicted: Rat, ) |
| 产品应用 | IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 12kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Glutaredoxin 1: 21-80/106 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the glutaredoxin family. The encoded protein is a cytoplasmic enzyme catalyzing the reversible reduction of glutathione-protein mixed disulfides. This enzyme highly contributes to the antioxidant defense system. It is crucial for several signalling pathways by controlling the S-glutathionylation status of signalling mediators. It is involved in beta-amyloid toxicity and Alzheimer's disease. Multiple alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Aug 2011]. Function: Has a glutathione-disulfide oxidoreductase activity in the presence of NADPH and glutathione reductase. Reduces low molecular weight disulfides and proteins. Subcellular Location: Cytoplasm. Similarity: Belongs to the glutaredoxin family. Contains 1 glutaredoxin domain. SWISS: P35754 Gene ID: 2745 Database links: Entrez Gene: 2745 Human Entrez Gene: 93692 Mouse Omim: 600443 Human SwissProt: P35754 Human SwissProt: Q9QUH0 Mouse SwissProt: P12864 Rabbit Unigene: 28988 Human Unigene: 712696 Human Unigene: 25844 Mouse Unigene: 1484 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Glutaredoxin 1) Polyclonal Antibody, Unconjugated (bs-23373R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
