| 产品编号 | bs-23762R |
| 英文名称 | Bik/NBK Rabbit pAb |
| 中文名称 | 促凋亡Bik蛋白抗体 |
| 别 名 | Apoptosis inducer NBK; BBC1; Bcl-2-interacting killer; BCL2 interacting killer; bhikhari; BIK; Bik-like killer protein; BIK_HUMAN; BIP 1; BIP1; BP 4; BP4; cb 60; NBK. |
| 研究领域 | 细胞生物 免疫学 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:200-2000 ELISA=1:500-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 18 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Bik/NBK: 10-90/160 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is known to interact with cellular and viral survival-promoting proteins, such as BCL2 and the Epstein-Barr virus in order to enhance programed cell death. Because its activity is suppressed in the presence of survival-promoting proteins, this protein is suggested as a likely target for antiapoptotic proteins. This protein shares a critical BH3 domain with other death-promoting proteins, BAX and BAK. Function: Accelerates programmed cell death. Association to the apoptosis repressors Bcl-X(L), BHRF1, Bcl-2 or its adenovirus homolog E1B 19k protein suppresses this death-promoting activity. Does not interact with BAX. Subunit: Interacts with RHBDL4/RHBDD1. Subcellular Location: Endomembrane system; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein (By similarity). Note=Around the nuclear envelope, and in cytoplasmic membranes. Post-translational modifications: Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism. SWISS: Q13323 Gene ID: 638 Database links: Entrez Gene: 638 Human Entrez Gene: 12124 Mouse Omim: 603392 Human SwissProt: Q13323 Human SwissProt: O70337 Mouse Unigene: 475055 Human |
| 产品图片 |
Sample:
Huvec(human) cell Lysate at 30 ug
Primary: Anti-Bik'NBK (bs-23762R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18kD
Observed band size: 22 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
