| 产品编号 | bsm-51379M |
| 英文名称 | CGAS Mouse mAb |
| 中文名称 | MB21D1 单克隆抗体 |
| 别 名 | C6orf150; D4; MB21D1; h-cGAS; CGAS_HUMAN; CGAS; cGAMP synthase; 2'3'-cGAMP synthase; Mab-21 domain-containing protein 1; 2.7.7.86; |
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Specific References (1) | bsm-51379M has been referenced in 1 publications.
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[IF=7.129] Xiaofei Wang. et al. Selenomethionine mitigate PM2.5-induced cellular senescence in the lung via attenuating inflammatory response mediated by cGAS/STING/NF-κB pathway. ECOTOX ENVIRON SAFE. 2022 Dec;247:114266 IF ; Mouse. 222
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| 研究领域 | 细胞生物 免疫学 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 7E3 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:500-5000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 59 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MB21D1/ C6orf150 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
MB21D1 (Mab-21 Domain Containing 1) is a Protein Coding gene. Diseases associated with MB21D1 include Aicardi-Goutieres Syndrome and Renpenning Syndrome. Among its related pathways are Innate Immune System and Cytosolic sensors of pathogen-associated DNA. GO annotations related to this gene include GTP binding and cyclic-GMP-AMP synthase activity. Function: Nucleotidyltransferase that catalyzes the formation of cyclic GMP-AMP (cGAMP) from ATP and GTP. Catalysis involves both the formation of a 2,5 phosphodiester linkage at the GpA step and the formation of a 3,5 phosphodiester linkage at the ApG step, producing c[G(2,5)pA(3,5)p]. Has antiviral activity by acting as a key cytosolic DNA sensor, the presence of double-stranded DNA (dsDNA) in the cytoplasm being a danger signal that triggers the immune responses. Binds cytosolic DNA directly, leading to activation and synthesis of cGAMP, a second messenger that binds to and activates TMEM173/STING, thereby triggering type-I interferon production. cGAMP can be transferred between cells by virtue of packaging within viral particles contributing to IFN-induction in newly infected cells in a cGAS-independent but TMEM173/STING-dependent manner (PubMed:26229115). (Microbial infection) Upon M.tuberculosis infection THP-1 cells knocked-out for this gene have impaired type-I interferon production (IF-1 beta), nor do they produce type-I IFN upon transfection with dsDNA (PubMed:26048138). Subcellular Location: Cytoplasm, cytosol. Tissue Specificity: Expressed in the monocytic cell line THP1. Similarity: Belongs to the mab-21 family. SWISS: Q8N884 Gene ID: 115004 Database links: Entrez Gene: 115004 Human Omim: 613973 Human SwissProt: Q8N884 Human Unigene: 658405 Human |
| 产品图片 |
25 ug total protein per lane of various lysates (see on figure) probed with CGAS monoclonal antibody, unconjugated (bsm-51379M) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
