| 产品编号 | bs-23674R |
| 英文名称 | LTA4H Rabbit pAb |
| 中文名称 | 白三烯A4水解酶抗体 |
| 别 名 | LKHA4_HUMAN; LTA4H; LTA-4 hydrolase; Leukotriene A(4) hydrolase; Tripeptide aminopeptidase LTA4H; 3.3.2.6; LTA4; LKHA4_RAT; |
| 研究领域 | 肿瘤 细胞生物 免疫学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse, (predicted: Human, Rat, Dog, Pig, Horse, Rabbit, Sheep, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 69 kDa |
| 检测分子量 | 69 |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LTA4H: 1-100/611 <Cytoplasmic> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Leukotrienes are a group of bioactive compounds which play important roles in immediate hyposensitivity reactions and inflammation. Leukotriene A4 hydrolase hydrolyzes an epoxide moiety of leukotriene A4 (LTA4) to form leukotriene B4 (LTB4). The enzyme also has some peptidase activity. Function: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Subunit: Monomer. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1 and isoform 2 are expressed in monocytes, lymphocytes, neutrophils, reticulocytes, platelets and fibroblasts. Post-translational modifications: Phosphorylation at Ser-416 inhibits enzymatic activity. Similarity: Belongs to the peptidase M1 family. SWISS: P09960 Gene ID: 4048 Database links: Entrez Gene: 4048 Human Omim: 151570 Human SwissProt: P09960 Human Unigene: 524648 Human Unigene: 104990 Rat |
| 产品图片 |
Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti- LTA4H (bs-23674R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 69 kD
Observed band size: 69 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LTA4H) Polyclonal Antibody, Unconjugated (bs-23674R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
