| 产品编号 | bsm-51301M |
| 英文名称 | LAMP1 Mouse mAb |
| 中文名称 | 溶酶体相关膜蛋白1（CD107）单克隆抗体 |
| 别 名 | CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane |
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Specific References (1) | bsm-51301M has been referenced in 1 publications.
111
[IF=7.464] Lu Zhang. et al. Southern rice black-streaked dwarf virus induces incomplete autophagy for persistence in gut epithelial cells of its vector insect. PLOS PATHOG. 2023 Jan;19(1):e1011134 CoIP ; Spodoptera frugiperda. 222
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| 研究领域 | 肿瘤 免疫学 神经生物学 信号转导 干细胞 细胞类型标志物 新陈代谢 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 2C5 |
| 交叉反应 | Human, |
| 产品应用 | WB=1:500-1000 ELISA=1:5000-10000 IF=1:20-200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LAMP1 |
| 亚 型 | IgG1,k |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation. Function: Presents carbohydrate ligands to selectins. Also mplicated in tumor cell metastasis. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=This protein shuttles between lysosomes, endosomes, and the plasma membrane. Post-translational modifications: O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. Similarity: Belongs to the LAMP family. SWISS: P11279 Gene ID: 3916 Database links: Entrez Gene: 3916 Human Entrez Gene: 16783 Mouse Omim: 153330 Human SwissProt: P11279 Human SwissProt: P11438 Mouse Unigene: 494419 Human Unigene: 16716 Mouse Unigene: 475822 Mouse Unigene: 40177 Rat |
| 产品图片 |
Sample:
Lane 1: Human HT1080 cell lysates
Lane 2: Human Hela cell lysates
Lane 3: Human U937 cell lysates
Primary: Anti-LAMP1 (bsm-51301M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 90-120 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
