| 产品编号 | bsm-41144M |
| 英文名称 | Procalcitonin(PCT) Mouse mAb |
| 中文名称 | 降钙素原单克隆抗体 |
| 别 名 | Calcitonin; CALCA; Calcitonin carboxyl-terminal peptide; CCP; Katacalcin; PDN-21; CALC1; |
| 研究领域 | 心血管 免疫学 信号转导 生长因子和激素 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 13 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Procalcitonin: 26-141aa |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Procalcitonin (PCT) is a peptide precursor of the hormone calcitonin, the latter being involved with calcium homeostasis. It arises once preprocalcitonin is cleaved by endopeptidase. It is composed of 116 amino acids and is produced by parafollicular cells (C cells) of the thyroid and by the neuroendocrine cells of the lung and the intestine. Function: The level of procalcitonin in the blood stream of healthy individuals is below the limit of detection (0.01 µg/L) of clinical assays. The level of procalcitonin rises in a response to a proinflammatory stimulus, especially of bacterial origin. It does not rise significantly with viral or non-infectious inflammations. With the derangements that a severe infection with an associated systemic response brings, the blood levels of procalcitonin may rise to 100 µg/L. In serum, procalcitonin has a half-life of 25 to 30 hours. Remarkably the high procalcitonin levels produced during infections are not followed by a parallel increase in calcitonin or a decrease in serum calcium levels. Subcellular Location: Secreted. DISEASE: Sepsis: Measurement of procalcitonin can be used as a marker of severe sepsis caused by bacteria and generally grades well with the degree of sepsis, although levels of procalcitonin in the blood are very low. PCT has the greatest sensitivity (85%) and specificity (91%) for differentiating patients with systemic inflammatory response syndrome (SIRS) from those with sepsis, when compared with IL-2, IL-6, IL-8, CRP and TNF-alpha. Evidence is emerging that procalcitonin levels can reduce unnecessary antibiotic prescribing to people with lower respiratory tract infections. Currently, procalcitonin assays are widely used in the clinical environment. Pneumonia: Procalcitonin levels may be useful to distinguish bacterial infections from nonbacterial infections. This may help guide antibiotic use, which can help save on cost and drug resistance. Kidney disease: Patients with chronic kidney disease and end-stage renal disease are at higher risk for infections, and procalcitonin has been studied in these populations, who often have higher levels. Procalcitonin can be dialyzed, and so levels are dependent upon when patients receive hemodialysis. While there is no formally accepted cutoff value for patients undergoing HD, using a value of greater or equal to 0.5 ng/mL yielded a sensitivity of 97-98% and a specificity of 70-96%. Hepatitis: PCT, possibly together with CRP, is used to corroborate the MELD score. Similarity: Belongs to the calcitonin family. SWISS: P01258 Gene ID: 796 Database links:
Entrez Gene: 796 Human Omim: 114130 Human SwissProt: P01258 Human Unigene: 37058 Human 降钙素(CT)是甲状腺滤泡旁细胞(C细胞)分泌的含有32个氨基酸的单链多肽，可使血钙、磷水平降低的一种多肽激素，脑组织和垂体含有降钙素，前列腺等其他组织也存在CT。主要用于C细胞增生和甲状腺髓样癌的诊断、也可用于其他类型的神经内分泌肿瘤的研究。 |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (human thyroid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Incubation with (Procalcitonin(PCT)) Monoclonal Antibody, Unconjugated (bsm-41144M) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.
4% Paraformaldehyde-fixed TT (H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Procalcitonin(PCT)) monoclonal Antibody, unconjugated (bsm-41144M) 1:200, 90 min at 37°C; followed by conjugated Goat Anti-Mouse IgG antibody (green, bs-60296G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
