| 产品编号 | bsm-33426M |
| 英文名称 | AMPK b1 Mouse mAb |
| 中文名称 | 腺苷单磷酸活化蛋白激酶β1单克隆抗体 |
| 别 名 | 5 AMP activated protein kinase subunit beta 1; AMPK; AMPK beta 1 chain; AMPKb; HAMPKb; PRKAB1; 5'-AMP-activated protein kinase subunit beta-1; AMP-activated protein kinase beta subunit; protein kinase, AMP-activated, noncatalytic, beta-1; AMPK beta-1 chain; 5'-AMP-activated protein kinase beta-1 subunit; AMPKb; AMPK subunit beta-1; AAKB1_HUMAN; AMPK b1; AMPK-b1. |
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Specific References (3) | bsm-33426M has been referenced in 3 publications.
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[IF=7.129] Xing Guo. et al. Microcystin leucine arginine induces human sperm damage: Involvement of the Ca2+/CaMKKβ/AMPK pathway. ECOTOX ENVIRON SAFE. 2023 May;256:114845 WB ; Human. 222
111
[IF=5.811] Han Xue. et al. Protective Effects of 6-Gingerol on Cardiotoxicity Induced by Arsenic Trioxide Through AMPK/SIRT1/PGC-1α Signaling Pathway. FRONT PHARMACOL. 2022 Apr;0:1298 WB ; Mouse. 222
111
[IF=2.843] Lili Jin. et al. A synthetic peptide AWRK6 ameliorates metabolic associated fatty liver disease: involvement of lipid and glucose homeostasis. Peptides. 2021 Sep;143:170597 WB ; Mouse. 222
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| 研究领域 | 细胞生物 免疫学 信号转导 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 3G2 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human AMPK b1 Protein: 118-226/272 |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex. [provided by RefSeq, Jul 2008]. Function: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3). Subunit: AMPK is a heterotrimer of an alpha catalytic subunit (PRKAA1 or PRKAA2), a beta (PRKAB1 or PRKAB2) and a gamma non-catalytic subunits (PRKAG1, PRKAG2 or PRKAG3). Interacts with FNIP1 and FNIP2. Tissue Specificity: Highly expressed in kidney, heart, white adipose tissue, lung and spleen. Post-translational modifications: Phosphorylated when associated with the catalytic subunit (PRKAA1 or PRKAA2). Phosphorylated by ULK1; leading to negatively regulate AMPK activity and suggesting the existence of a regulatory feedback loop between ULK1 and AMPK. Similarity: Belongs to the 5'-AMP-activated protein kinase beta subunit family. SWISS: Q9Y478 Gene ID: 5564 Database links: Entrez Gene: 5564 Human Entrez Gene: 19079 Mouse Omim: 602740 Human SwissProt: Q9Y478 Human SwissProt: Q9R078 Mouse Unigene: 6061 Human Unigene: 726001 Human Unigene: 458152 Mouse Unigene: 3619 Rat |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
U87MG(Human) Cell Lysate at 30 ug
U251(Human) Cell Lysate at 30 ug
Primary: Anti- AMPK b1 (bsm-33426M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 38 kD
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- AMPK b1 (bsm-33426M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti- Mouse IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 38 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AMPK b1) Monoclonal Antibody, Unconjugated (bsm-33426M) at 1:800 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
