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MouseAnti-Cas9 Mouse mAb  antibody (bsm-52042M)
~~~促销,代码KXJ230206~~~
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说明书: 50ul  100ul  
50ul/1580.00元
100ul/2500.00元
大包装/询价

产品编号 bsm-52042M
英文名称 Cas9 Mouse mAb
中文名称 CRISPR/Cas9单克隆抗体
别    名 CAS9_STRP1; CRISPR-associated endonuclease Cas9/Csn1; EC:3.1.-.-; SpCas9; SpyCas9; CRISPR-Cas9/Csn1; csn1; SPy_1046;  
抗体来源 Mouse
克隆类型 Monoclonal
克 隆 号 1E8
产品应用 WB=1:500-2000 ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 160 kDa
检测分子量
性    状 Liquid
浓    度 1mg/ml
免 疫 原 Recombinant fragment corresponding to Streptococcus pyogenes CRISPR-Cas9 (N terminal) 
亚    型
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.

Function:
CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.

Subunit:
Monomer. Binds crRNA and tracrRNA.

Similarity:
Belongs to the CRISPR-associated protein Cas9 family.
Subtype II-A subfamily.

Database links:

SwissProt: Q99ZW2 Streptococcus pyogenes



产品图片
Sample: Lane 1: CRISPR-Cas9 transfected 293 cells lysate Lane 2: Non-transfected 293 cells lysate Primary: Anti-CRISPR-Cas9 (bsm-52042M) at 1/5000 dilution Secondary: Goat Anti-Mouse IgG - HRP at 1/5000 dilution Predicted band size: 160 kD Observed band size: 150 kD
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