| 产品编号 | bsm-52066R |
| 英文名称 | Cytokeratin 9 Recombinant Rabbit mAb |
| 中文名称 | 细胞角蛋白9重组兔单抗 |
| 别 名 | CK 9; CK-9; CK9; Cytokeratin-9; Cytokeratin9; EPPK; K1C9_HUMAN; K9; Keratin 9; Keratin; Keratin type I cytoskeletal 9; Keratin-9; Keratin9; KRT 9; KRT9; type I cytoskeletal 9. |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 4F8 |
| 交叉反应 | Human, Rat, (predicted: Mouse, ) |
| 产品应用 | WB=1:500-1000 IHC-P=1:100-500 IHC-F=1:50-200 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human Cytokeratin 9 protein (300-450aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes the type I keratin 9, an intermediate filament chain expressed only in the terminally differentiated epidermis of palms and soles. Mutations in this gene cause epidermolytic palmoplantar keratoderma. [provided by RefSeq, Jul 2008] Function: May serve an important special function either in the mature palmar and plantar skin tissue or in the morphogenetic program of the formation of these tissues. Plays a role in keratin filament assembly. Tissue Specificity: Expressed in the terminally differentiated epidermis of palms and soles. DISEASE: Defects in KRT9 are a cause of palmoplantar keratoderma epidermolytic (EPPK) [MIM:144200]; also abbreviated as EHPPK. EPPK is a dermatological disorder characterized by diffuse thickening of the epidermis on the entire surface of palms and soles sharply bordered with erythematous margins. Some patients may present with knuckle pads, thick pads of skin appearing over the proximal phalangeal joints. Similarity: Belongs to the intermediate filament family. SWISS: P35527 Gene ID: 3857 Database links: Entrez Gene: 3857 Human Omim: 607606 Human SwissProt: P35527 Human Unigene: 654569 Human |
| 产品图片 |
Sample:
Lane 1: Rat Ear tissue lysates
Lane 2: Human A431 cell lysates
Primary: Anti-Cytokeratin 9 (bsm-52066R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kDa
Observed band size: 60 kDa
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
