| 产品编号 | bsm-52137R |
| 英文名称 | phospho-CDC37 (Ser13) Recombinant Rabbit mAb |
| 中文名称 | Hsp90辅助伴侣分子CDC37重组兔单抗 |
| 别 名 | Cdc37(phospho S13); Cdc37(phospho Ser13); p-Cdc37(phospho S13); CDC 37; CDC37_HUMAN; Hsp90 co-chaperone Cdc37; CDC37A; Hsp90 chaperone protein kinase-targeting subunit; p50Cdc37; Hsp90 co-chaperone Cdc37, N-terminally processed; cell division cycle 37, HSP90 cochaperone; P50CDC37; |
| 产品类型 | 磷酸化抗体 重组兔单抗 |
| 研究领域 | 细胞生物 细胞周期蛋白 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 8A1 |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:1000-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 44 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human CDC37 around the phosphorylation site of Ser13: EV(p-S)DD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is highly similar to Cdc 37, a cell division cycle control protein of Sacchromyces cerevisiae. This protein is a molecular chaperone with specific function in cell signal transduction. It has been shown to form complex with Hsp90 and a variety of protein kinases including CDK4, CDK6, SRC, RAF-1, MOK, as well as eIF2 alpha kinases. It is thought to play a critical role in directing Hsp90 to its target kinases. [provided by RefSeq, Jul 2008] Function: Co-chaperone that binds to numerous kinases and promotes their interaction with the Hsp90 complex, resulting in stabilization and promotion of their activity. Subcellular Location: Cytoplasm. Post-translational modifications: Constitutively sumoylated by UBE2I. Similarity: Belongs to the CDC37 family. SWISS: Q16543 Gene ID: 11140 Database links: Entrez Gene: 11140 Human Entrez Gene: 12539 Mouse SwissProt: Q16543 Human SwissProt: Q61081 Mouse |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate:
-): C6, (+): C6 + Calyculin A
(100nM, 30min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 50 kDa
Observed MW: 50 kDa
Blocking buffer: 5% NFDM/TBST
Primary Ab dilution: 1:2000
Primary Ab incubation condition: 2 hours at
room temperature
Secondary Ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: (-): HeLa, (+): HeLa + Okadaic A
(100nM,60min) + Calyculin A (100nM,60min)
Protein loading quantity: 20 μg
Exposure time: 30 s
Predicted MW: 50 kDa
Observed MW: 50 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
