| 产品编号 | bsm-54625R |
| 英文名称 | SOX1 |
| 中文名称 | 转录因子SOX-1重组兔单抗 |
| 别 名 | SOX1_HUMAN; Transcription factor SOX-1; SRY-box transcription factor 1; |
| 研究领域 | 发育生物学 神经生物学 干细胞 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 23C9 |
| 交叉反应 | (predicted: Human, Mouse, Rat, ) |
| 产品应用 | WB=1:500-1000 IHC-P=1:100-500 ICC=1:50-100 IF=1:50-100 （石蜡切片需做抗原修复）
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 39 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human SOX1 protein (100-200aa) |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Sox genes comprise a family of genes that are related to the mammalian sex determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. Sox genes encode putative transcriptional regulators implicated in the decision of cell fates during development and the control of diverse developmental processes. The highly complex group of Sox genes cluster at least 40 different loci that rapidly diverged in various animal lineages. At present, 30 Sox genes have been identified. Members of this family have been shown to be conserved during evolution and to play key roles during animal development. Some are involved in human diseases, including sex reversal. Function: Transcriptional activator. Subcellular Location: Nucleus. Tissue Specificity: Mainly expressed in the developing central nervous system. Post-translational modifications: Sumoylation inhibits the transcriptional activity. Similarity: Contains 1 HMG box DNA-binding domain. SWISS: O00570 Gene ID: 6656 Database links: Entrez Gene: 6656 Human Entrez Gene: 20664 Mouse Omim: 602148 Human SwissProt: O00570 Human SwissProt: P53783 Mouse Unigene: 202526 Human Unigene: 39088 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded Human Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SOX1 Monoclonal Antibody, Unconjugated(bsm-54625R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
Paraformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with SOX1 Monoclonal Antibody, Unconjugated(bsm-54625R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit(Rabbit, SP-0023) and DAB (C-0010) staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
