| 产品编号 | bsm-52238R |
| 英文名称 | STAT5b Recombinant Rabbit mAb |
| 中文名称 | 信号转导和转录激活因子5b重组兔单抗 |
| 别 名 | Transcription factor STAT5B; Signal transducer and activator of transcription 5B; STA5B_HUMAN; STAT5. |
| 研究领域 | 肿瘤 染色质和核信号 信号转导 转录调节因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | |
| 克 隆 号 | 12A1 |
| 交叉反应 | (predicted: Human, ) |
| 产品应用 | WB=1:1000-2000 IP=1:20-100
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 90 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human STAT5b protein, around 500-600aa |
| 亚 型 | |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein mediates the signal transduction triggered by various cell ligands, such as IL2, IL4, CSF1, and different growth hormones. It has been shown to be involved in diverse biological processes, such as TCR signaling, apoptosis, adult mammary gland development, and sexual dimorphism of liver gene expression. This gene was found to fuse to retinoic acid receptor-alpha (RARA) gene in a small subset of acute promyelocytic leukemias (APLL). The dysregulation of the signaling pathways mediated by this protein may be the cause of the APLL. [provided by RefSeq, Jul 2008] Subunit: Forms a homodimer or a heterodimer with a related family member. Binds NR3C1. Interacts with NCOA1, NMI and SOCS7. Interacts (via SH2 domain) with INSR. Subcellular Location: Cytoplasm. Nucleus. Note=Translocated into the nucleus in response to phosphorylation. Post-translational modifications: Tyrosine phosphorylated in response to signaling via activated KIT, resulting in translocation to the nucleus. Tyrosine phosphorylated in response to signaling via activated FLT3; wild-type FLT3 results in much weaker phosphorylation than constitutively activated mutant FLT3. Alternatively, can be phosphorylated by JAK2. Phosphoryation at Tyr-699 by PTK6 or HCK leads to an increase of its transcriptional activity. Dephosphorylation on tyrosine residues by PTPN2 negatively regulates prolactin signaling pathway. Similarity: Belongs to the transcription factor STAT family. Contains 1 SH2 domain. SWISS: P51692 Gene ID: 6777 Database links: Entrez Gene: 6777 Human Entrez Gene: 20851 Mouse Omim: 604260 Human SwissProt: P51692 Human SwissProt: P42232 Mouse Unigene: 595276 Human Unigene: 34064 Mouse Unigene: 54486 Rat |
| 产品图片 |
Blocking buffer: 5% NFDM/TBST
Primary ab dilution: 1:1000
Primary ab incubation condition: 2 hours at
room temperature
Secondary ab: Goat Anti-Rabbit IgG H&L
(HRP)
Lysate: HeLa, K562, PC-12, A20
Protein loading quantity: 20 μg
Exposure time: 10 s
Predicted MW: 90 kDa
Observed MW: 90 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
